Microarray is one such technology which enables the researchers to investigate and address issues which were once thought to be nontraceable. Over the past few years, this powerful technology has been used to explore transcriptional profiles and
Microarray is one such technology which enables the researchers to investigate and address issues which were once thought to be nontraceable. Over the past few years, this powerful technology has been used to explore transcriptional profiles and genome differences for a variety of microorganisms, greatly facilitating our understanding of microbial metabolism. With the increasing availability of complete microbial genomes, DNA microarrays are becoming a common tool in many areas of microbial research, including microbial physiology, pathogenesis, epidemiology, ecology, phylogeny, and pathway engineering. One can analyze the expression of many genes in a single reaction quickly and in an efficient manner. DNA Microarray technology helps in the identification of new genes and in knowing about their functioning and expression levels under different conditions, comparative genomics, and SNPs identification. This chapter has outlined the principle of microarray technology, types of microarray, their basic protocols, and applications.